JOURNAL OF CHILEAN CHEMICAL SOCIETY

Vol 63 No 3 (2018): Journal of the Chilean Chemical Society
Original Research Papers

CHIRAL CHROMATOGRAPHIC ANALYSIS AND ABSOLUTE STEREOCHEMISTRY OF RACEMIC CHLORPHENERAMINE USING CHIRAL HPLC-CD-OR AND TD-DFT CALCULATIONS

Marcelo A. Muñoz
Instituto de Ciencias Químicas, Facultad de Ciencias, Universidad Austral de Chile
Published September 12, 2018
Keywords
  • HPLC-CD-OR,
  • circular dichroism detection,
  • optical rotation detection,
  • DFT calculations

Abstract

A chiral HPLC analysis combined with time-dependent density functional theory (TD-DFT) calculations was used for the assignment of the absolute configuration (AC) of both enantiomers of the antihistamine drug chlorpheniramine. Circular dichroism and optical rotation detections coupled to the chromatographic system (HPLC-CD-OR) permitted the on-line measurement of the CD spectra and [α]670 values, while TD-DFT at the B3LYP/TZVP//B3LYP/VDZ(P) and B3LYP/ aug-cc-pVDZ//B3LYP/VDZ(P) levels of theory for CD and OR, respectively, delivered theoretical chiroptical properties in the gas and solution states. Both calculations were consistent in predicting the correct combination of CD spectra and [α]670 sign and therefore permitted the absolute configuration (AC) assignment of the optical isomers. The AC proposed using this methodology agreed with a previous X-ray study.

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