JOURNAL OF CHILEAN CHEMICAL SOCIETY

Vol 63 No 3 (2018): Journal of the Chilean Chemical Society
Original Research Papers

A REVIEW ON DERIVATIVE UV-SPECTROPHOTOMETRY ANALYSIS OF DRUGS IN PHARMACEUTICAL FORMULATIONS AND BIOLOGICAL SAMPLES REVIEW

Vivekkumar K. Redasani
Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research
Priyanka R. Patel
Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research
Divya Y. Marathe
Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research
Suraj R. Chaudhari
Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research
Atul A. Shirkhedkar
Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research
Sanjay J. Surana
Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research
Published September 12, 2018
Keywords
  • Derivative UV-Spectrophotometry,
  • First Order Derivative spectra,
  • Second Order Derivative spectra,
  • Third Order Derivative spectra,
  • Fourth Order Derivative spectra and area under curve

Abstract

The review article deals with theoretical aspects of Derivative UV-Spectrophotometry. The method gains significance using the first and second derivative of the transmission spectra with respect to wavelength. Generated optical derivatives are compared to the known numerical derivatives. The derivative spectra from 1st to 4th are consequently discussed. This provides valuable insight into the uses and limitations of this technique for chemical analysis. Measurement techniques and methods of obtaining derivative spectra are discussed. The degree of polynomial fit on the smoothness of derivative spectra and signal-to-noise ratio is described. Application of UV derivative spectrometry for determination of single and multicomponent analysis is shown. Derivative spectrophotometry possibly improves the selectivity and sensitivity of determination which has been illustrated.

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